Question
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1. By definition, Disinfectants are applied only to 2. Chemicals that have a Phenol Coefficient' greater than one are: (1 p a. less effective than phenol but cannot be applied to tissues b. equal in effectiveness to phenol c. more effective than phenol but must applied only to tissues d more effective than phenol e. less effective than phenol 3. List two features of the disk diffusion technique for antibiotic testing that have been standandized so that the test is applicable in laboratories/clinics around the globe. (2 pts) 4. The process that some bacteria and lower eukaryotes use that reverses thymine-thymine dimers in their DNA that has been caused by ultraviolet radiation is called: (1pt) 5. The laboratory narrative for the Physical Control' lab listed some ressons that bacteris do not grow below their minimam growth temperature and above their maxmum growth temperatun List one of these reasons for each temperature limit.(2 pts) At or below the Minimum Growth Temperature At or below the Maximum Growh Temperature 6. List any two of the six factors (given on page 2 of the laboratory narrative) than may affect the zom diameter when using the Disk Diffusion assay to determine antibiotic susceptbility, (2pas) 2.
Explanation / Answer
1. Non living objects to destroy microbes.
Disinfectants are anti microbials applied only to inanimate objects.
2. d) more effective than phenol
Disinfectant to be tested is compared with phenol on a standard microbe. Phenol coefficient greater than 1 are more effective disinfectants than phenol whereas less than 1 are less effective disinfectants.
3. 1.Disk diffusion technique for antibiotics tests the effectiveness of antibiotics on specific micoorganism.
2. Highly effective antibiotics will produce a wide range of no bacterial growth while an in effective antibiotic show no change in the surrounding bacterial concentration at all.
3. Circumferance of clear zones correlate MIC of antibiotic for that bacteria.
4. Photoreactivation Repair.