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Predict where each mutant protein will go (as in, final destination), based on t

ID: 264224 • Letter: P

Question

Predict where each mutant protein will go (as in, final destination), based on the set of sorting sequences it has.

Hint: think about what set of sorting sequences the normal, unmutated protein would have, then think about what sorting sequences the protein would have after the changes are made. Then, figure out where a protein with these new sorting sequences would end up.

a cytoskeletal protein, with MTS added to it

Cyt-P450 enzyme, with the ER-RS removed

Hint: we learned about the function of P450 in the slides.

Oligosaccharyl transferase, with the ER-SS and ER-RS removed

Hint: oligosaccharyl transferase adds the initial oligosaccharide (initial glycosylation)

DNA polymerase, with the NLS swapped out and replaced with ER-RS

glucocerebrosidase, with no M6P tag

ER

cytoplasm

golgi

secreted

stuck forever inside a vesicle

nucleus

mitochondria

      -       A.       B.       C.       D.       E.       F.       G.   

a cytoskeletal protein, with MTS added to it

      -       A.       B.       C.       D.       E.       F.       G.   

Cyt-P450 enzyme, with the ER-RS removed

Hint: we learned about the function of P450 in the slides.

      -       A.       B.       C.       D.       E.       F.       G.   

Oligosaccharyl transferase, with the ER-SS and ER-RS removed

Hint: oligosaccharyl transferase adds the initial oligosaccharide (initial glycosylation)

      -       A.       B.       C.       D.       E.       F.       G.   

DNA polymerase, with the NLS swapped out and replaced with ER-RS

      -       A.       B.       C.       D.       E.       F.       G.   

glucocerebrosidase, with no M6P tag

A.

ER

B.

cytoplasm

C.

golgi

D.

secreted

E.

stuck forever inside a vesicle

F.

nucleus

G.

mitochondria

Explanation / Answer

Predict where each mutant protein will go (as in, final destination), based on t