In the yeast-two hybrid system, interaction of two proteins is confirmed by what
ID: 266722 • Letter: I
Question
In the yeast-two hybrid system, interaction of two proteins is confirmed by what?
transcription of the reporter gene (lacZ)
attachment of the bait protein to the binding domain of the activator gene
attachment of the target protein to the activation domain of the activator gene
separation of the binding and activation regions of the activator gene in different vectors
transcription of the reporter gene (lacZ)
attachment of the bait protein to the binding domain of the activator gene
attachment of the target protein to the activation domain of the activator gene
separation of the binding and activation regions of the activator gene in different vectors
Explanation / Answer
Attachment of the bait protein to the binding domain of the activator gene – The bait is the fusion protein which binds to the DNA binding domain. The confirmation of the protein protein interaction takes place when the bait protein binds to the activator gene