To know which RNA polymerase has transcribed a certain gene Add a toxin to diffe
ID: 267361 • Letter: T
Question
To know which RNA polymerase has transcribed a certain gene
Add a toxin to differentially inhibit RNA polymerases and quantify isolated mRNA
Tag RNA polymerase with a radiolabeled signal and monitor the product using EMSA
Isolate mRNA and reverse transcribe it to cDNA
Quantify the DNA using southern blot and then amplify using PCR
Add a toxin to differentially inhibit RNA polymerases and quantify isolated mRNA
Tag RNA polymerase with a radiolabeled signal and monitor the product using EMSA
Isolate mRNA and reverse transcribe it to cDNA
Quantify the DNA using southern blot and then amplify using PCR
Explanation / Answer
B. Tag RNA Polymerase with a radiolabeled signal and monitor the product using EMSA.
EMSA can deduce the interactions between molecules so, radiolabeled enzyme can be found associated with the sequence it is going to transcribed.