MasteringGenexy MasteringGenex e How will Yuc ×? a Your Orders ×? a Amazon.com ×
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MasteringGenexy MasteringGenex e How will Yuc ×? a Your Orders ×? a Amazon.com ×? a Amazon.com a Amazon.com-x" a Amazon.com ×? How to take Zachy CSecure https://session.masteringgenetics.com/myct/itemView?assignmentProblemID-5710135 ?Homework #13 due May07-Lect21-22 Problem 17.26 9 of 14> Part B How will you create loss-of-function alleles of the mouse genes? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all. Reset Help flanking an essential Hox7 Loss-of-function alleles of Hoxa7 and Hoxb7 could be created by performing gene knockouts for each gene. The gene knockout would be performed by creating a transgene that contains DNA corresponding to the internal region of an essential Hoxi wilh a selectable marker in the place of an exon. A selectable marker would be included outside the DNA Injection of this construct into cells and selection for the selectable marker and against the selectable marker would yield clones containing the knockout of negative These cells would then be used to generate mice that carry the knockout construct in egg their ES germ line at least one Hox7 gene both Hox7 genes Submit Part CExplanation / Answer
The gene knockout would be performed by creating a transgene that contains DNA corresponding to the internal regions of an essential Hox exon with a positive selectable marker in the place of an exon. A negative selectable marker would be included outside the DNA flanking an essential Hox7 exon. Injection of this construct into the mouse ES cells and selection for the germline selectable marker and against the at least one Hox7 gene selectable market would yield clones containing the knockout of both Hox7 genes. These sperm cells would then be used to generate mice that carry the knockout construct in their egg cells.