I\'m only stuck on Question 6 \"Why is it important to sequence your DNA molecul
ID: 269109 • Letter: I
Question
I'm only stuck on Question 6 "Why is it important to sequence your DNA molecule from both ends (the 5’ and 3’)". The rest of the worksheet is for refrence and background information.
SEQUENCING WORKSHEET Name: 1. List one methad that is used to sequence DNA malecules? 10 pts 2. What is a chain terminator? 10 pts 3. How do chain terminators cause DNA synthesis to stop? 10 pts 4. How do chain terminators see a point mutation in the sequence of a DNA molecule (will it be seqenced cs the mutation & how will you know that it is a mutation?)? 20 pts 5. What is the difference between maual sequencing and automated sequencing? 20 pts 6. wahy is it important to sequence your DNA molecule from both ends (the 5 and 3? 10 pts 7. List one reason why someone would want to identify the sequence of a particular DNA mclecule. 10 pts. B. List one chain terminator used to treat AIDS. 5pts b. List one chain terminctor used to treat herpes. 5 ptsExplanation / Answer
Q1). Dideoxy sequencing:
1. Sanger sequencing or dideoxy sequencing uses dideoxynucleotides (ddNTPs) and primers.
2. Deoxyribonucleic acid (dideoxynucleotides, ddNTP's) structure is similar to deoxyribonucleic acid (DNA), but it is lack of one oxygen atom present next to nucleotide pairing. Deoxyribonucleic acids are used as inhibitors of chain-elongation by DNA polymerase.
3. The fluorescently labeled or radioactive ddNTP’s are introduced into a growing strand of DNA to terminate the elongation process.
4. A series of DNA fragments of different sizes are produced by this process, the size of these fragments is measured by electrophoresis.
5. In each fragment, the base present at its end is known as its dideoxynucleotide, which was used to terminate the reaction.
6. By arranging these fragments in order based on their size, the DNA sequence can be framed out.
7. This technique is used to determine the DNA sequence, and it gives specific results.
Q6).
As the DNA polymerase moves in 3’ to 5’ direction, it also moves in the opposite direction to form the lagging strand. This is important to complete the replication at a faster rate.