Answer by true or false 1)The introns: Are smaller than the exons Are common in
ID: 272556 • Letter: A
Question
Answer by true or false
1)The introns:
Are smaller than the exons
Are common in bacteria
Correspond to the region 5’utr of the messenger RNA
2)A cloning vector:
Has its own origin of replication
A polylinker
An antibiotic resistance gene
A promoter allowing the expression of the inserted DNA fragment
3)The double helix b of the DNA:
At a step of 3.4 nm
Has a diameter of 23.7 nm
Is composed of two parallel strands
4)After hydration by northern blot if two hybridization bands of different sizes give the same intensity of hybridization it means:
There is the same number of nucleic acid molecule
There is the same mass of nucleic acid molecule
5)Prokaryotic RNA polymerase allows synthesis:
Messenger RNA
Transfer RNA
Ribosomal RNA
6)The transcribed strand of a gene corresponds:
Corresponds to the same sequence of the messenger RNA
Is used as a template by the RNA polymerase
Is read in the 3’ to 5’ direction by the polymerase
7)The RNA is hydrolyzed:
When the pH is acidic
When the temperature increases
8)The restriction card:
Is deduced from the restriction profile
Can be deduced from the nucleotide sequence of the fragment
9)The large 50S subunit of a prokaryotic ribosome contains
The 16S RNA
The 23S RNA
The 5.8 S RNA
10)Lactose operon:
Is present in all living organisms
Has only two coding regions
11)Complementary DNA synthesis requires
An oligo DT primer
DNA polymerase
A DNA matrix
Reverse transcriptase
DNase 1
12)Some RNase can:
Cut double-stranded RNA
Cut single-stranded RNA
Recognize specific bases
13)The transcription unit:
Contains the tata box
Contains the initiation codon of the translation
14)TFIIB factor in eukaryotes:
Is a translation factor
Is a termination factor
Is initiation factor
15)TAQ polymerase:
Is used in cloning
Is used in PCR
Comes from a bacteria resistant to high temperature
16 )An enhancer region is able to fix an activator
17)The total messenger RNA can be separated from the other RNA:
By cesium chloride gradient centrifugation
By agarose gel electrophoresis
By affinity chromatography
18)If 50 micrograms in 1 ml of dna have an absorbance of 260 nm the same amount of arn will have an absorbance
Lower
Superior
19)An expression vector has:
Its own origin of replication
A resistant gene to an antibiotic
A promoter allowing the expression of the inserted DNA fragment
20)Sequencing by the Sanger method:
Requires the use of desoxyadenosinetriphosphate
Requires the use of reverse transcriptase
Requires the use of didesoxyadenosinetriphosphate
Explanation / Answer
Answer 1. False. Intron are larger than exon.
False intron doesn't find in bacteria.
False intron are present within gene
Answer 2. True cloning vector has its own origin of replication.
True Cloning vector's multicloning site also known as polylinker
True closing vector has resistance gene
False cloning vector doesn't have promoter so it can not express
Answer 3. False DNA complete its turn at 3.4 nm and each step is 0.34nm.
False. Has diameter of 2 nm
False DNA consist of two anti parallel strands
Answer 4. In Northern blotting amount of nucleic acid was tagged so there is same number of nucleic acid