Complete the last coloumn and state why these reactions occured. For example, in
ID: 272873 • Letter: C
Question
Complete the last coloumn and state why these reactions occured. For example, in tube 3, there was a larger gas bubble because there was a presence of magnesium ions which meant the enzyme elonase was able to function.
METABOLISM (FERMENTATION) What are the expected results/did you get them. Tube A B C Initial Reading Final Reading Volume of the gas bubble created If not why and Explain 1 5 ml phosphate buffer 1 5 ml phosphate buffer 5 ml yeast 1mL 1.5mL 0mL 2 5 ml phosphate buffer 5 ml glucose 5 ml yeast 1.5mL 6.5mL 5mL 3 5 ml of 0.1 M MgSO4 5 ml glucose 5 ml yeast 1mL 8mL 7mL 4 5 ml of 50 mM EDTA 5 ml glucose 5 ml yeast 1mL 6mL 5mL 5 5 ml of 100 mM EDTA 5 ml glucose 5 ml yeast 1.5mL 2.5mL 1mL 6 5 ml of 250 mM EDTA 5 ml glucose 5 ml yeast 1mL 1mL 0mL 7 5 ml pyruvate/EDTA 5 ml glucose 5 ml yeast 1mL 8.5mL 7.5mL 8 5 ml phosphate buffer 5 ml of 0.1 M lactose 5 ml yeast 1mL 2mL 1mL 9 5 ml phosphate buffer 5 ml of 0.1 M galactose 5 ml yeast 1.5mL 2mL 0.5mLExplanation / Answer
Fermentation pathway in yeast converts glucose to CO2 which is measured as gas bubble formation in the tubes. In the 1st tube only phosphate buffer is there no carbohydrates. Hence this must be a control. So no gas bubble. In 2nd tube carbohydrate is there , so glucose converted to co2 . Gas bubble formation. In 3rd tube glcose + mg2+ is present. Presence of Mg+2 speeds the fermentation process and hence the volume of gas bubble is large. In 4th, 5th and 6th tubes EDTA is present along with Glucose . the concentration of EDTA increases in each tube and the gas bubble formation decreases in each tube respectively. This is because presence of EDTA chelates the ions responsible for growth of yeast like zinc, iron etc. thus fermentation process is inhibited . So as EDTA concentration incraeses, gas bubble decreases respectively in each tube. Pyruvate fermentation is different pathway and hence co2 is formed. Yeast does not have enzymes to break lactose and galactose and hence the formation of co2 is low or nil in last 2 tubes.