Map Sapling Learning macmillan learning The following statements describe SDS-PA
ID: 273575 • Letter: M
Question
Map Sapling Learning macmillan learning The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements. Assume that SDS-PAGE is performed under reducing conditions. Protein-SDS complexes have similar mass to charge ratios, therefore separation is by size. Sodium dodecyl sulfate binds proteins, resulting in protein-SDS complexes that are similar in size. Proteins are separated in a polyacrylamide gel matrix. Protein-SDS complexes migrate toward the negative electrode. Smaller proteins migrate faster through the polyacrylamide gel. Proteins are visualized using a dye that binds to the gel matrix, but not to proteinsExplanation / Answer
The SDS complex have similar masses to charge ratios, therefore separation by size
SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) is commonly used for the separation of proteins based on their molecular weight
Smaller protein migrate faster through the polyacrylamide gel
Proteins are separated in a polyacrylamide gel matrix