Microbiology, i am a bit confused right now. i have been given an assigment to i
ID: 276976 • Letter: M
Question
Microbiology, i am a bit confused right now. i have been given an assigment to identify a bacteria i am workring with and answer some questions using scholarly sources. the only issue is i am finding it hard to firgure out which source to use as they contradict each other sometimes.
The Bacteria is Bacillus Subtilis(well known)
I have the following questions, although i have answered them all; i still need clarification and another source. please attach a link to all the sources used so i can take a look at them. thanks
Question My answers Glucose Fermentation Phenotypes (Growth? With Acid? With Gas) grows sometimes, (idk the rest cant find sources) Term to describe metabolism:(chemoheterotroph, photoheterotroph, chemoautotroph, chemolithotroph, photoautotroph) chemoheterotropic (im sure of this but some sources have stated otherwise) Media that best supports the growth of this organism: Bacilus Subtilus rich media and selective sounds most likely (some sources have said glucose and minimal for some reason) Temperature requirements for optimal growth: Term to describe this organism with respect to temperature requirements: 37 degrees (but some have say lower at 30 degrees) Optimal Salt Concentration. Is the organism a halophile or halotolerant, or neither? halotolerant Lactose Fermentation Phenotypes (Growth? With Acid? With Gas) usually does not ferment lactose. (idk the rest cant find sources)
Explanation / Answer
Source of glucose fermentation: streptococcus bovis- Gram positive bacteria in humans associated with urinary tract infections, Enterobacter aerogenes, Serratia marcescens, Shigella flexneri, Citrobacter frundii.
Photoheterotrophic- Oriental hornet
Chemoautotrophic- Nitrosomonas, methanogens, iron bacteria
Chemoautotrophic- Thiobacillus ferroxidans
Photoautotrophs- pleurocapsales, RhodobacterMedium for B. subtilis- peptone yeast extract
medium, nutrient agar medium, Difco sporulation medium, Luria bertani broth
Optimal temperature is 30- 40 °c.
Lactose fermentation phenotypes- klebsiella pneumoniae, Escherichia Coli, Serratia, Enterobacteriaceae.