Topic: Gel Electrophoresis and Restriction Mapping: 2 What is the purpose of gel
ID: 277100 • Letter: T
Question
Topic: Gel Electrophoresis and Restriction Mapping: 2 What is the purpose of gel electrophoresis? 3 How does gel electrophoresis allow us to isolate and study specific DNA (or other macromolecules) - in other words what chemical characteristics does gel electrophoresis take advantage of that allow us to study macromolecues2 4 What is a plasmid? 5. Where are plasmids normally found in nature? 6. What type of genes are typically found on plasmids? 7. Are the genes found on plasmids necessary for survival of the organism 8. What is the difference between a restriction site and a restriction endonuclease? 9 Which restriction endonucleases were used to cut the plasmid DNA that we loaded into the gels? If a circular piece of DNA has three restriction sites for a particular restriction enzyme, into how many fragments will that restriction enzyme cut the DNA? 10. If a linear piece of DNA has three sites for a particular restriction enzyme, into how many fragments will that restriction enzyme cut the DNA? 11.Explanation / Answer
Ans 2 :
The technique of Gel electrophoresis is used to seperate different biomolecules such as DNA , RNA and proteins.
The principle for this process is the seperation of molecules on the basis of size and charge.
DNA is seperated on the basis of size , and the proteins are seperated on the basis of size and charge , since different proteins have different charges.