If your stock concentration of ampicillin is 100 mg/ml, how much would you add t
ID: 277743 • Letter: I
Question
If your stock concentration of ampicillin is 100 mg/ml, how much would you add to 500 ml of LB Agar (or Luria Agar, LA) to have a final ampicillin concentration of 50 ug/ml? Show your work. (2 points) 9. If you used the LA (Luria Agar) that you prepared in the previous q pour your plates, and if each plate contained 25 ml of LA, what is the approximate amount of ampicillin (grams) in each plate? Use the information from the previous question to help you. Show your work. (2 points) broth) to have a final broth concentration of 50 ug/ml? Show your work. (2 points) 10. How much of the same ampicillin stock would you add to 5 ml of LB (LuriaExplanation / Answer
(8)
Stock=100 mg/mL,
we need =50ug/mL = 0.05mg/mL
Volume= 500mL
C1V1= C2V2
(stock) (required)
V1=C2V2/C1 =(0.05 x 500)/100=0.25mL
0.25mL of stock solution made up to a total of 500 mL diluent gives concentration of 50 ug/mL
(9)
0.05mg/mL
So 25 mL LB agar contain = 25 x 0.05 =1.25mg = 1.25 x 10^-3g
(10)
V1=C2V2/C1 =(0.05 x 5)/100=0.0025mL = 2.5uL