Part IX. (18 points) Enzymes. The following plot was made from data obtained by

Question

Part IX. (18 points) Enzymes. The following plot was made from data obtained by measuring the initial rates of the succinate dehydrogenase under standard assay conditions. The concentration of succinate was varied in one set of experiments in the absence of an inhibitor(-I) and in another in the presence of 8 the inhibitor (+). 8 -4 -2 0 2 4 6 8 10 12 14 16 18 succinate (A) Does the succinate dehydrogenase reaction obey Michaelis-Menten kinetics? Explain the basis for your answer. es be caa se the ets are lincaa San?C,bat. ear stays cres the Y aris at the Sem (B) What is the mode of action of inhibitor used in these experiments? Justify Piak your answer ompehtve nhbte be cause a Stays the saneuhe n chages (C) Calculate the Km value for succinate with this enzyme with and without inhibitor. Show your work

Explanation / Answer

Intercept on X-axis = - 1/Km

- 1/Km, apparent (I+) = 2

-1/Km (I-) = 4

Intercept on Y axis = 1/Vmax = 2

I- : Slope = Km/Vmax

I+ : Slope = Kmapp / Vmax

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