Describe how the original spectral flow cytometry works and the advantage and di
ID: 279043 • Letter: D
Question
Describe how the original spectral flow cytometry works and the advantage and disadvantages of it
Fluorescence (500-800nm Flat-top beam: Ensures quantitatve data (fluorescence intensity) even if the stream line of each cell changes in the microfluidic channel D & SSC Prism array Flowlcell chip Flat top opticS 488 nm Micro lens array 32ch PMT 638 nm Spot size -100 x 6 ?m Astigmatic optics & Quadrant photodio Dual lasers PMT Channel 1 5 10 15 20 25 30 32 500 550 600 650 700 750 800 Wavelength [nm]Explanation / Answer
Answer:
Spectral flowcytometry is the advancement of the original conventional technique with the use of spectrograph and multichannel detector. The advantages is the use of fluorescent nanocrystals that have wide absorption bands and narrow emission bands along with detectors that are electronic with higher sensitivity. In addition, the side scatter light is collected into spectrograph and whole light is scattered that which is caught on high resolution detector. One of the main disadvantage in using using flow cytometer is the slower analyses rates.