Two arrestin genes (?-arrestin 1 and 2) have been characterized and are expresse
ID: 279418 • Letter: T
Question
Two arrestin genes (?-arrestin 1 and 2) have been characterized and are expressed ubiquitously. Over the past several years, the development of knockout mice in which the genes for B-arrestin 1 and 2 have been inactivated have greatly aided the study of the physiological and cellular roles of these multifunctional proteins. To better understand the role of B-arrestin 1 and 2 in the desentitization of the ß2-AR small interfering RNA (RNAi) were used to specifically suppress the expression of ß arrestin 1 or 2 or both. The results are shown in the figure below. · siRNAExplanation / Answer
The two siRNA molecules are ?-arr1 and ?-arr2. As shown in the figure, when ?-arr1 is presented to the ?-arrestin 1 and ?-arrestin 2, the ?-arrestin 1 is suppressed and silenced to a certain extent. On the other hand, when ? arr-2 is applied to both, ?-arrestin 2 is drastically silenced when compared to ? arrestin 1 before.
When both the siRNA molcules are exposed to ?arrestin 1 and 2, both of them are suppressed, however, ?arrestin 1 is silenced more than when applied only ?arr-1. But, ? arrestin 2 is not suppressed much.
So, the specificity of the ? arr 1 indicates that it can suppress ?-arrestin 1 to a certain extent and inhibit the suppression of the ? arrestin 2.
Specificty of ?-arr 2 indicates that it can suppress ? arrestin 1 more than ? arr1.