A. Ouabain can disrupt ATP binding to the Na+/K+ pump. B. ATP is required for tr
ID: 280652 • Letter: A
Question
A. Ouabain can disrupt ATP binding to the Na+/K+ pump.
B. ATP is required for transport of Na+ and not for transport of K+.
C. The ATP binding site of the Na+/K+ pump is located on the intracellular domain of the pump.
D. The ATP binding site of the Na+/K+ pump is located on the extracellular domain of the pump.
9) AT ATP Ouabain Ouabain Was Nat Was K+ trans- present present present present insideoutside inside outside trans- cells? cells?cells? cells? ported? ported? Yes Yes No No 5 Yes Yes Yes Yes No Yes 0 0 No No No No No No Yes Yes No No No No No Yes No Yes Yes Yes No No No No Yes Yes Yes No No No Yes No 2 The sodium-potassium (Na+/K+) pump functions like an anti-porter transporting Na+ and K+ across membranes using ATP. This protein spans the membrane with intracellular and extracellular domains. It has a binding site for Nat, K+, and ATP. An experiment was conducted to determine the locations of these binding sites. Artificial cells were created and incubated in buffers containing ATP, ouabain (or oubain), Na, and K+ in varying combinations inside and outside of the cell as indicated in the chart. The transport of Na+ and K+ was measured to determine activity of the Na+/K+ pump. Which of the following conclusions is supported by the data?Explanation / Answer
C. The ATP binding site of the Na+/K+ pump is located on the intracellular domain of the pump.