Table1.1 RF values of common photosynthetic pigments Line number Band distance f

Question

Table1.1                 RF values of common photosynthetic pigments

Line number

Band distance from origin (cm)

Solvent distance from the origin (cm)

RF Value

Photosynthetic Pigment name

1

1.5

5.5

0.27272

Xanthophylls

2

3

5.5

0.54545

Chlorophyll b

3

4

5.5

0.72727

Chlorophyll a

4

5

5.5

0.90909

Carotene

Line number

Band distance from origin (cm)

Solvent distance from the origin (cm)

RF Value

Photosynthetic Pigment name

1

1.5

5.5

0.27272

Xanthophylls

2

3

5.5

0.54545

Chlorophyll b

3

4

5.5

0.72727

Chlorophyll a

4

5

5.5

0.90909

Carotene

Explanation / Answer

      Rf means therelative front which we obtain in paper chromatography.       This gives the mobility ofthe sample in the two phases i.e., mobile phase and the immobilephase.       Rf iscalculated as :                                             distancetravelled by solvent                                   Rf =    distance travelled by solute       Paper chromatography isused to separate the molecules.       From this information wecan say that the mobility or migration of carotene is faster i.e.,it along with the mobile phase       while the movement of xanthophylls is very less.       We can also say thatcarotene movement is followed by chlorophyll a, chlorophyll b andthen xanthophylls followed it .       I hope this has servedyour purpose.

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