Here is your original DNA; assume the top strand is the template strand: 3\' - T
ID: 281386 • Letter: H
Question
Here is your original DNA; assume the top strand is the template strand:
3' - TAC CTT TTA TAG TAG ATA CCA CAA AGG ACT- 5'
5' - ATG GAA AAT ATC ATC TAT GGT GTT TCC TGA- 3'
1. Transcribe the above DNA (make the complementary mRNA to the template strand).
2. Translate the mRNA into an amino acid sequence.
3. Write the anticodons for the amino acids in the sequence (tRNA).
4. Change the nucleotide in position #18 to C.
5. Repeat steps 1, 2 & 3.
6. How has this change affected the amino acid sequence?
7. What type of mutation is this? Is it harmful? Explain.
8. Now remove nucleotides 16 through 18.
9. Repeat steps 1, 2 & 3.
10. What type of mutation is this?
11. Do all mutations result in a change in the amino acid sequence?
12. Are all mutations considered bad? Explain.
13. The above sequence codes for a genetic disorder called cystic fibrosis (CF). When A is changed to G in position #18, the person does not have CF. When T is changed to C in position #17, the person has the disorder. How could this have originated?
Explanation / Answer
Answer=
1] Transcription of DNA
3' - TAC CTT TTA TAG TAG ATA CCA CAA AGG ACT- 5' - template DNA strand
5' - AUG GAA AAU AUC AUC UAU GGU GUU UCC UGA-3'- mRNA strand
=======================================================================================
2. Translation of the mRNA into an amino acid sequence.
5' - AUG GAA AAU AUC AUC UAU GGU GUU UCC UGA-3'- mRNA strand
3' - met - Glu - Asn - Ile - Ile - Tyr - Gly - Val - Ser -5' - amino acid sequence
======================================================================================
3. Write the anticodons for the amino acids in the sequence (tRNA).
3' - met - Glu - Asn - Ile - Ile - Tyr - Gly - Val - Ser -5' - amino acid sequence
5' - AUG GAA AAU AUC AUC UAU GGU GUU UCC UGA-3'- mRNA strand
- UAC CUU UUA UAG UAG AUA CCA CAA AGG ACU -anticodon sequence in tRNA.
=======================================================================================