Mixtures of amino acids can be analyzed by separating the mixture into its compo

Question

Mixtures of amino acids can be analyzed by separating the mixture into its components throughon Map dft exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfonate (-SO3) groups flow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate groups on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin For each pair of amino acids listed, determine the order of elution from a cation exchanger in pH 7.0 buffer Amino Acid Pair Elut Asp and Arg Lys and Leu Ala and Glu Gly and Met Trp and Thr Elutes Second Arg Leu Glu Met Trp Asp Lys Ala Thr

Explanation / Answer

Amino acid pair elutes first elutes second Asp and Arg Asp Arg Lys and Leu Leu Lys Ala and glu Glu Ala Gly and Met Gly Met Trp and Thr Trp Thr

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