QUESTION The anomerio ourbon in woug O Partolpatos in glycosldic bonds O Is bond
ID: 300845 • Letter: Q
Question
QUESTION The anomerio ourbon in woug O Partolpatos in glycosldic bonds O Is bonded to nitrogenated bases in nucleotides O Comesponde to ponition 1 (0) in suger molecule O All of the above QUESTION 2 Cellulose and o-amylose are both made up of glucose dimers, but they use diferont types of olycosidic bonds ?Tue O False QUESTION 3 A solution contains a mixture of Proteins A (Size: 50 kDa.pl + 7.5) and Protein B (Size: 50 kDa, pl 3.5) Which technique would be best to separate these proteins from one another based on the provided information? 0 Size Exclusion Chromatography ? Dialysis Affinity Chromatography (via affinity tag) on Exchange ChromatogrsExplanation / Answer
1] Answer-- all of the above
A glycosidic bond or linkage is a type of covalent bond that joins a sugar molecule to another group which may or may not be another carbohydrate.
The anomeric carbon is a stereocenter. This carbon can be either alpha or beta depending on the direction of the OH group attached to it.
2] Answer- True
Amylose and cellulose are linear polymers of glucose linked with 1,4- bonds.
Cellulose is derived from D-glucose units, which condense through B [ 1 to 4 ] glycosidic bonds.
Amylose is a polysaccharide made of a-D- glucose units, bonded through a [ 1 to 4] glycosidic bonds.
3] Answer- Ion-exchange Chromatography
This method is used to separate proteins carrying a charge. The pl or isoelectric point of a protein is defined as the pH at which it has no net charge. When the pH.> pl, the protein has a net negative charge and when the pH < pl, the protein has a net positive charge.
Since the above protein molecules are of the same size but differ in the charge they carry, the above-mentioned method will be the best to separate them.