Diagnostic Molecular Biology You perform a post-transplant short-tandem repeat e

Question

Diagnostic Molecular Biology You perform a post-transplant short-tandem repeat engraftment study by sequence-based typing. An example of informative versus non-informative markers is presented below: 5. Marker Recipient Alleles Donor Alleles D851179 D21511 075820 30 29 30 12 28 10 In this example, D21511 would NOT be informative D7S820 are both informative since the two samples differ in the alleles present. in the figure below, you have five markers for donor (top) and recipient (bottom). Based on since both samples contain 30, whereas D851179 and example, which markers will be informative, and which will not? 8787 Markers D351358 THO1 D135317 D165539 D251338 Informative/Not Informative

Explanation / Answer

Markers Informative/Non informative D3S1358 Informative; Donor and recepient differ in alleles TH01 Non informative; Both have 7 allele as common D13S317 Non informative; Both have 12 allele as common D16S539 Informative D2S1338 Informative

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