Insert Design Layout References Mailings Review View Arial Styles Style Pane b.
ID: 301599 • Letter: I
Question
Insert Design Layout References Mailings Review View Arial Styles Style Pane b. The Py subunit of the trimeric G protein has been implicated in increased metastasis of cancer cells. Before metastasis occurs researchers have observed increased calcium levels in the cells. Explain why researchers equate increasing Ca*" levels in the cell to GBy signaling by outlining how this protein would cause Ca++ release. Rubric (5): Outline ALL the steps in the pathway that would result i, ??++ release. we e process you ou then use that antibody to suppress metastasis! That would be awesome! Pick a component of the pathway you outlined in "c and inhibit a specific aspect of its function using a function blocking (FY!... it needs to be directed toward a protein!). Be sure to conclude with why this would decrease signaling from that specific protein. All plausible answers are correct! Rubric (Oj: normal protein agonist (forskolin) in the presence of your antibody. Now, think of measurable outcomes for the GBy side of the pathway you could measure (sorry you cannot use Ca release or metastasis). What outcome did you pick for the GBy side and what do you expect to see if the cells are treated with forskolin and your antibody when compared to forskolin treatment alone? R treatment result Page 4 of 5 1890 words FocusExplanation / Answer
1. G?? belongs to the G protein coupled receptors .(GPCR)
Activation of GPCR would result in inactivation os Ca+ channels through the G?? receptors.
'Thus there is decreased Ca2+ output if GPCR is activated.
2.As we can see, scientists have found that that there is an increase in the Ca2+ ion release before the metastasis.
So there is a direct connection between the Ca2+ ion release and the metastasis.
This would definitely help us to produce the antibody that would inhibit the metastasis.
there should be introduction of such antibody which results iin the activation of G?? whioch will ultimately lead to inhibition of Ca2+ ion release.
3.Forskolin actually activates the enzyme adenyl cyclase , which increases the amount of cAMP in the cells.
If the forskolin is supposed to work with the antibody, it should help the antiibody in activaton of G??.