Before adding color reagent, sample tube #2 contained 0.35 mL of a 1:100 dilutio

Question

Before adding color reagent, sample tube #2 contained 0.35 mL of a 1:100 dilution of sample Y plus 0.65 mL of buffer A, and sample tube #3 contained 1 mL of a 1:1000 dilution of sample Z. Which of the following conclusions can you draw from the data in this assay?

Sample X is less concentrated than sample Y.

Sample Y is less concentrated than sample X.

Sample Z is less concentrated than sample Y.

Sample X and Y have approximately the same concentrations.

Sample Y and Z have approximately the same concentrations.

Explanation / Answer

Any information about a sample X is not mentioned anywhere in the questions so the details are not enough to have the precise answer but from the given facts the answer should be sample Z is less concentrated than Y. because even after addition of a buffer which is almost twice of the volume of the sample volume, Y managed to give a higher absorbance than sample Z which means the concentration is higher. when the buffer is added usually sample is further diluted eventhough the final volumes are the same.

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