In your effort to understand what may be wrong with individuals carrying a mutan
ID: 3165325 • Letter: I
Question
In your effort to understand what may be wrong with individuals carrying a mutant pretendin gene, you decide to try a little bioinformatics With the help of a computer program that allows you to compare a protein sequence to all other known protein sequences to find regions of high similarity between them, you find that pretendin is very similar to another protein called madeupin, which binds to kinetochores in mitotic chromosomes. Furthermore, you discover that the now famous L>E pretendin mutation that you've been investigating so far happens within an amino acid sequence of pretendin that is almost identical toa region of madeupin known to be critical for its ability to bind kinetochores and facilitate the latching of spindle microtubules to chromosomesExplanation / Answer
Answer,
The normal pretendin molecules are expected to to found in the cytosol as well as the nucleus of the cell which are in the G1 phase of growth.This is because cells in G1 phase have just came out of mitosis and pretendin is to be found in the nucleus and cytosol.
In case of cells in metaphase phase, major portion of pretensin will occcur with kinetochores complex, associated with the metaphase chromosome and will exclusively present at metaphse plate.
In case of anaphase, the pretendiin is expected to be found towards the opposite poles of the cell as the sister chromatids are pulled away from each other.
In case of point muation (Leu->E), the pretendin will lose its ability to bind the kinetochore complex. Hence, the mutant protein is expected to be found in nucleus and cytosol in all phases of the cell cycle.
The chromosomes will be stationed at the metaphase plate.