If there are two fragments of RNA say strand 1 and strand 2 to be \"stitched\" t

Question

If there are two fragments of RNA say strand 1 and strand 2 to be "stitched" together (both sequence 1 and 2 going in 5' to 3' direction),

for example, strand 1 is 5' AAGAUUCAUC 3'

and

strand 2 is 5' CGAGUGCCAUGCCUGGUCUAAGGAGGUAUGAGUAAUGUUGUAAAAUAGUUAGUCUAC 3',

would the stitching be done as

strand 1+strand 2 -

5' AAGAUUCAUCCGAGUGCCAUGCCUGGUCUAAGGAGGUAUGAGUAAUGUUGUAAAAUAGUUAGUCUAC 3'

or

strand 2+strand 1? -

5' CGAGUGCCAUGCCUGGUCUAAGGAGGUAUGAGUAAUGUUGUAAAAUAGUUAGUCUACAAGAUUCAUC 3'

I understand RNA is always made in 5' to 3' direction but both seem to be a valid 5' to 3' growth. The question asks to stitch the first 9 nucleotides of a sequence (strand 1) to sequence 2. Please explain as which of the two is the right way to stitch and why.

Explanation / Answer

Answer: - I think the stitching can be done in both ways as both seem to be the valid ways to join the mRNA sequences together. I don't think it matters whether you put sequence 1 before of sequence two before as long as they are correctly oriented in 5'-3' direction.

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