Please I need help on this lac operon partial diploid genotypes, I have solution
ID: 323726 • Letter: P
Question
Please I need help on this lac operon partial diploid genotypes, I have solution, thorough STEP BY STEP explanation will be appreciated.
For the following lac operon partial diploid genotypes, indicate with a (+) or (- ) whether enzymatic activity would be detected for -Galactosidase and/or Permease when lactose is absent and when lactose is present. Solution is given below could you explain how to get them?
A.) I+ P+ Oc Z+ Y- / Is P+ O+ Z- Y+
B.) I- P+ O+ Z+ Y+ / I+ P+ O+ Z+ Y-
C.) I+ P- Oc Z+ Y- / I+ P+ O+ Z- Y+
D.) I- P+ Oc Z+ Y- / I+ P+ O+ Z- Y+
E.) Is P- Oc Z+ Y+ / I+ P+ O+ Z+ Y-
F.) I+ P- O+ Z+ Y- / I- P+ OC Z- Y+
Lactose Absent
Lactose Present
b-Gal
Permease
b-Gal
Permease
A
+
-
+
-
B
-
-
+
+
C
-
-
-
+
D
+
-
+
+
E
-
-
-
-
F
-
+
-
+
Lactose Absent
Lactose Present
b-Gal
Permease
b-Gal
Permease
A
+
-
+
-
B
-
-
+
+
C
-
-
-
+
D
+
-
+
+
E
-
-
-
-
F
-
+
-
+
Explanation / Answer
E. Coli breaks down lactose using two enzymes, beta-galactosidase and permease. Beta-galactosidase is encoded by the LacZ gene and permease is encoded by LacY gene.
To solve a lac operon problem we need to consider the following: