The mutagen 5-bromouracil (5-BU) is a base analog of thymine. When added to the

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The mutagen 5-bromouracil (5-BU) is a base analog of thymine. When added to the growth medium of cells, 5-BU is assimilated into nucleotide triphosphates and incorporated by DNA polymerase into DNA. 5-BU can cause base pair substitutions when a shift in its tautomeric state alters its normal base pairing behavior. The DNA sequence of a single strand of a portion of a wild-type gene and that of 5 spontaneous mutant alleles of the gene are shown below. wild-type: 5? GCACGGTCATGCGCTCAGG 3? allele #1: 5? GCACCGTCATGCGCTCAGG 3? allele #2: 5? GCACGGTCATGTGCTCAGG 3? allele #3: 5? GCGCGGTCATGCGCTCAGG 3? allele #4: 5? GCACGGTCATGCGCCCAGG 3? allele #5: 5? GCACGGTCTTGCGCTCAGG 3? Part A Which of the alleles might have been induced by 5-BU? Enter your answer as a number (not word). If there is more than one possible allele, enter your answer in numerical order, without spaces or commas. For example: 1235

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