Book : Alcamo\'s Laboratory Fundamentals Of Microbiology ( Biochemical Character

Question

Book : Alcamo's Laboratory Fundamentals Of Microbiology

( Biochemical Characteristics of Bacteria ) ( Lab 26 page 223-233 )

A) Carbohydrate fermentation

Dextrose (Glucose), Yellow     Acidic

Lactose, Orange    Basic

Sucrose, Yellow     Acidic

Maltose, Orange   Basic

B) Starch Digestion

- Amylase present because (clear / light)

- Brown Streak around Bacteria (+)

C) Catalase Test

            - Nutrient Agar Plates

            - Oxygen Bubbles present = Catalase

D) DNase Digestion

            - No zone of clearing because it cannot digest the dna-cloudy

E) Hydrogen sulfide

            - Aerobic

            - Positive (can produce Cysteine)

F) IMViC Series

     - Indole

- (Inoculate trypticase soy broth)

     - Methyl Red

- (Inoculate mR

Explanation / Answer

1) presence of substrate.

2) To get accurate results and identification of organism pure culture is performed

3) Indole test is used to determine the ability of an organism to spilt amino acid tryptophan to form the compound indole. Tryptophan is hydrolysed by tryptophanase to produce three possible end products

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