You have a fluorescein (Fl)-labeled (green-dye) antibody to the m heavy chain an

Question

You have a fluorescein (Fl)-labeled (green-dye) antibody to the m heavy chain and a rhodamine (Rh)-labeled (red-dye) antibody to the d heavy chain. Describe the fluorescent antibody staining pattern of the following B cell maturational stages, assuming that you can visualize both membrane and cytoplasmic staining: (so tell me if there is staining and what color(s) and where the staining is taking place [on the cell membrane or in the cytoplasm])

1) Progenitor B cell (pro-B cell)

2) Precursor B cell (pre-B cell)

3) Immature B cell

4) Mature B cell

5) Plasma cell before any class switching has occurred

Explanation / Answer

1.Pro B cell(proginator B cell)

both Fl and Rh are not expressed.

2.Pre b cell(Precursor B cell)

Fl expressed and Rh not. It expressed on m heavy chain.

3.Immature B cell

Fl dont expresses only Rh expressed. It expressed on m heavy chain

4.Mature B cell

Fl and Rh both gets expressed.Both express on heavy chain

5.Plasma cell before any class switching has occured

both not get expressed

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