Indicate your method(s) of choice for determining the structure of each of the f
ID: 36560 • Letter: I
Question
Indicate your method(s) of choice for determining the structure of each of the following proteins:
1)Protein for which no information about homologs is available, but 3-D crystals can be easily obtained
2) Protein for which no information about homologs is available, and 3-D crystals are not obtained, but 2-D crystals can be easily obtained
3)Protein for which no information about homologs is available, and neither 3-D nor 2-D crystals can be obtained
4)Protein for which crystals are not obtained but a homolog from other organism with a 50% level of similarity is known
NOTE: In some cases two approaches may be equally applicable, separately or in combination; in such cases, you need to indicate both
The methods available are:
X-ray crystallography
NMR spectroscopy
Cryo-electron microscopy and electron crystallography
Homology modeling
A priori predictions
Explanation / Answer
Answer:
1)X-ray crystallography,NMR spectroscopy,Cryo-electron microscopy and electron crystallography
2)Circular dichroism,Homology modeling
3)Isotope exchange and protection techniques
4)A priori predictions,Homology modeling