The membrane potential across the inner mitochondrial membrane is -60mV (negativ
ID: 37680 • Letter: T
Question
The membrane potential across the inner mitochondrial membrane is -60mV (negative inside), and the external side of this membrane has a ph that is 1.4 units below th pH on the internal side of this membrane(i.e.More acidic externally.
A. how much free energy is produced if one mole of H+ is transported into the mitochondrail matrix under these conditions?
B. Under steady-state condtions in a cell, the free energy change for hydrolysis of ATP is approximately -11.5 kcal/mol.How many moles of H+ must be transported across the membrane to provide energy for the sythesis of one mole of ATP?
Explanation / Answer
A) -
but, the proton motive force across the mitochondrial membrane should be 50 kJ/mol for ATP to be synthesized.
B) approximately 3.4 H+ ions needed to be pumped across the membrane to synthesize one ATP molecule.