The following sequence was found to occur on a mRNA molecule: 5\'-CCUUACACAGGAAA
ID: 3944 • Letter: T
Question
The following sequence was found to occur on a mRNA molecule: 5'-CCUUACACAGGAAACACGUAUGACCAUUGAGGCUUGAACAA-3'What amino acid sequence is represented here followingtranslation?
For the above mRNA sequence, identify the bases thatcorrespond to the Shine-Dalgarno sequence. Explain.
Describe how the amino acid sequence would change if thetwenty-first base (U) were mutated to G 5'-CCUUACACAGGAAACACGUAUGACCAUUGAGGCUUGAACAA-3'
What amino acid sequence is represented here followingtranslation?
For the above mRNA sequence, identify the bases thatcorrespond to the Shine-Dalgarno sequence. Explain.
Describe how the amino acid sequence would change if thetwenty-first base (U) were mutated to G
Explanation / Answer
The synthesis of proteinsfrom m-RNA is known as translation. The bases in m-RNA areread in the form of codons. Three bases togetherconstitute a codon that corresponds to the particularaminoacid. For translation to getinitiated on m-RNA, the initiation codon is AUG that codes forMethionine aminoacid. In the given sequence thisinitiating codon is present after 19 nucleotides. From this AUG codon theaminoacids are added accrodingly that correspond to thecodon. The mechanism oftranslation stops when there is a stop codon which is either UAA,UAG or UGA. Basing on this theaminoacid sequence for the given sequence is as: 5'-C C U U A C A C A G G AA A C A C G U AUG ACC AUU GAG GCU UGA ACA A-3' N-terminal - Met Thr Ile Glu Ala - C-terminal Hence the amino acidesequence for the given m-RNA sequence is Met - Thr -Ile - Glu - AlaShinedalgarno sequence is the sequence on m-RNA that corresponds to 16sr-RNA in the 30S subunit of ribosome. This sequence ispurine rich sequence that is present before the initiatingcodon. This sequnece is aninitiation signal of 4 to 9 purine residues which is 8 to 13basepairs to the 5' side of theinitiation codon. Basing on thisinformation, the Shine dalgarno sequence in the above m-RNAsequence is identified as AGGAAA. 5'-C C U U A CA C AGGAAA C A C G U AUG ACC AUU GAGGCU UGA ACA A-3'.
If the21st U is mutated to G, then there would be noinitiation codon in the given sequence and hence there will notbe any amino acids thatcorresponds to the particular sequence. Which means that noprotein is synthesized from the given m-RNAsequence. As there is noinitiation codon protein synthesis will not be initiated and thecorresponding aminoacids will not be coded. 5'-C C U U A C A C AG G A A A C A C G U AUG ACC AUU GAG GCU UGA ACA A-3' 5'-C C U U A C A C A G G AA A C A C G U AGG ACC AUU GAG GCU UGA ACA A-3' Instead if you wantto know what aminoacid corresponds to AGG it isArg.
Hope youare satisfied with the answer.
Shinedalgarno sequence is the sequence on m-RNA that corresponds to 16sr-RNA in the 30S subunit of ribosome. This sequence ispurine rich sequence that is present before the initiatingcodon. This sequnece is aninitiation signal of 4 to 9 purine residues which is 8 to 13basepairs to the 5' side of theinitiation codon. Basing on thisinformation, the Shine dalgarno sequence in the above m-RNAsequence is identified as AGGAAA. 5'-C C U U A CA C AGGAAA C A C G U AUG ACC AUU GAGGCU UGA ACA A-3'.
If the21st U is mutated to G, then there would be noinitiation codon in the given sequence and hence there will notbe any amino acids thatcorresponds to the particular sequence. Which means that noprotein is synthesized from the given m-RNAsequence. As there is noinitiation codon protein synthesis will not be initiated and thecorresponding aminoacids will not be coded. 5'-C C U U A C A C AG G A A A C A C G U AUG ACC AUU GAG GCU UGA ACA A-3' 5'-C C U U A C A C A G G AA A C A C G U AGG ACC AUU GAG GCU UGA ACA A-3' Instead if you wantto know what aminoacid corresponds to AGG it isArg.
Hope youare satisfied with the answer.