Please answer the following question Short answer. Please fill in the blanks bel
ID: 41893 • Letter: P
Question
Please answer the following question Short answer. Please fill in the blanks below the question with your answer. tRNA. Please answer the following 3 questions based on the figure to the left. 5 u c 3 What would be the result if the mRNA had a different base instead of the C? Why? What is often found in the anticodon at the position where the base G is located? What would be the effect ifthe Uin the mRNA were a different Short answer. Please provide short answers for the next three questions. (two points each) Two RNA molecules were isolated from a eukaryotic cell and their sequence was determined, and regions of the RNA molecules were numbered as shown below. m A A A m 1 2 3 4 5 6 7 8 The investigators were able to find this following DNA that had been the template for the RNA molecules: DNA Q hy does transcript ve a different sequence than the DNA sequence? Where in the cell was transcript#2 when it was isolated from the cellm How do you know? clear Q: What specific kind of transcripts are both and #2?Explanation / Answer
For the 1st question:
- If mRNA had different base instead of C then also there will be no difference in binding as this is wobble position. At this position the binding is not as tight as on other position so there is possibility of formation of unusual bonding.
- Position where G located is known as wobble base So probably it would be Inosine so that it could bind to any of the base.
- If there is any other base than U on that location in mRNA then binding of tRNA and mRNA would not take place.
For the second question:
- Transcript #1 is mature mRNA which is formed after post transcriptional modifications ie. 5' capping, 3' polyadenylation and splicing(removal of introns). The sequence differ from DNA template because DNA had introns(non-coding region) and exons(coding region) both but mature mRNA consist only exons.
- Transcript #2 would be present in nucleus only because it will come to cytoplasm only after post transcriptional modifications which takes place in nucleus only.
- Transcript #1 is Mature mRNA i.e. ready for translation and Transcript #2 is pre mRNA molecule which need to be processed before translation.