For routine slides, bacteria are attached or \"fixed\" to theslide by: a. precoa
ID: 4716 • Letter: F
Question
For routine slides, bacteria are attached or "fixed" to theslide by:a. precoating slides with a thin film ofalcohol
b. Air drying, then addingEthanol to cover slide
c. Air drying, removing allliquid.
d. Air drying, then passingslide several times through a flame.
e. use loop to stick bacteriaonto slide
Which of the following steps are NOT done for preparing a slidefrom an agar plate culture of bacteria? [Check ALL that are NOTdone]
a. Use a coverslip to hold bacteria inplace.
b. Transfer several"loopfulls" of bacteria to slide
c. Spread sample over slideand fix.
d. Flame loop beforesampling from plate.
e. Add a 1/2 drop ofdistilled water to slide.
For routine slides, bacteria are attached or "fixed" to theslide by:
a. precoating slides with a thin film ofalcohol
b. Air drying, then addingEthanol to cover slide
c. Air drying, removing allliquid.
d. Air drying, then passingslide several times through a flame.
e. use loop to stick bacteriaonto slide
Which of the following steps are NOT done for preparing a slidefrom an agar plate culture of bacteria? [Check ALL that are NOTdone]
a. Use a coverslip to hold bacteria inplace.
b. Transfer several"loopfulls" of bacteria to slide
c. Spread sample over slideand fix.
d. Flame loop beforesampling from plate.
e. Add a 1/2 drop ofdistilled water to slide.
Explanation / Answer
1.) d. Air drying, then passing slide severaltimes through a flame Forthe preparation of a bacterial slide, first the slide is cleanedwith alcohol, then a loop full of culture is takenand a smear is prepared. Thissmear is air dried and passed over the flame for several times forheat fixation. Tosuch heat fixed smear, the stain is added and then the cover slipis placed over it.2.) b. Transfer several "loopfulls" of bacteriatoslide
For the preparation of slide, 1/2 drop os distilled water is placedon the slide and then the loop that is to be used is flamed before sampling from the plate. Then a single loop full of culture is taken but not severalloopfulls. This loop full of culture is then placed on the slide and a smearis prepared and heat fixed
a. Use acoverslip to hold bacteria inplace.
Acoverslip is used to flatten the water drop and allows to focuswithh high power very close to the specimen. Thiscoverslip confines the specimen to a single plane and therebyreduces the amount of necessary focussing. Hence,a coverslip is not used to hold the bacteria in place but is usedto for the above explained purpose. Thereforethese two options are not done for preparing a slide from agarplate. Hopeyou are satisfied with the answer