Preparation of four calibration g for two species (not food dyes!) that have ove

Question

Preparation of four calibration g for two species (not food dyes!) that have overlapping spectra produces the following set of extinction coefficients. epsilon_A lamnda_max for A = 7.8 M^-1 Cm^-1 epsilon_A lambda_max for B = 2.6 M^-1 cm^-1 epsilon_B lambda_max for A = 1.5 M^-1 cm^-1 epsilon_B lambda_max for B = 5.2 M^-1 cm^-1 Analysis of an unknown solution produces absorbance values of: 0.45 lambda_max for B 0.75 lambda_max for A What are the molar concentrations of the unknown A and B (Be sure to show all your w no credit will be given otherwise.)

Explanation / Answer

According to Beer-Lambert's law,

absorbance = molar extinction coefficient (L/mol.cm) * path length (=1cm) * concentration (mol/L)

A = *l*C

C = A/ * l

C = 0.45 / 7.8 *1

C = 0.058 mol/L

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