An enzyme linked inmanosorbent assay requires radioactive subitrale. B a radioac
ID: 483801 • Letter: A
Question
An enzyme linked inmanosorbent assay requires radioactive subitrale. B a radioactive standard for binding to the antibody. aromatic amino a ids, D) an antibody bends the protein of interest 8. ch of the following amino acids would be last to elute at pH 8.0 from an anion exchange Co glutamic acid E) glycine trying to separate which are listed below.by gel filtration chromatography Which of the proteins from the column? A) cytochrome c 012 kDa) B) RNA polymerase (99 kDa) C glutamine synthetase (621 kDa) D) interferon-T 34 kDa) E) hemoglobin (62 kDa) 20. Disulfide bonds can be cleaved using A) iodoacetate, B) dansyl chloride. C (B-ME) D) trypsin. E phenylisothiocyanate. True/False. State whether the statement is TRUE orFALSE. If FALSE, please correct the statement using complete answers with full sentences. 2 pnt each 21. A solution containing a weak acid (pK. 7.5) and its conjugate base at pH of8.5 has a good capacity to buffer the addition ofacid. 22. The insolubility of nonpolar molecules in water is due to the large positive entropy, which is the result of water molecules forming an ordered network surround ng nonpolar molecules. 23. Eukaryotic genes often contain regions that are transcribed and later excised from the RNA, called 24. In the peptide Trp ser val valine is at the C-terminus.Explanation / Answer
17
An enzyme-linked immunosorbent assay requires
A)a radioactive substrate.
B)a radioactive standard for binding to the antibody
.C)aromatic amino acids
.D)an antibody that binds the protein of interest.-----------------answer
E)a catalytic antibody.
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18. Which of the following amino acids would be last to elute at pH 8.0 from an anion-exchangecolumn?
A)lysine
B)alanine
C)glutamic acid---------------------answer
D)asparagine
E)glycine
Answer:C
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19 .You are trying to separate five proteins, which are listed below, by gel filtrationchromatography.Which of the proteins will elute first from the column?A)cytochrome c (12 kDa)
B)RNA polymerase (99 kDa)
C)glutamine synthetase (621 kDa)----------answer
D)interferon-g(34 kDa)
E)hemoglobin (62 kDa)
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20 Disulfide bonds can be cleaved using
A)iodoacetate
.B)dansyl chloride.
C)2-mercaptoethanol (b-ME).-------------answer
D)trypsin
.E)phenylisothiocyanate
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Enzyme-linked immunosorbent assay (ELISA) : it is also known as a catalyst immunoassay (EIA), is a biochemical system utilized for the most part in immunology to recognize the nearness of an immunizer or an antigen in a specimen.
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Glutamic corrosive :it is a -amino corrosive that is utilized as a part of the biosynthesis of proteins. It contains a -amino gathering (which is in the protonated NH3+ shape under organic conditions), a -carboxylic corrosive gathering (which is in the deprotonated CO2 frame under natural conditions), and a side chain carboxylic corrosive, characterizing it as a polar contrarily charged (at physiological pH), aliphatic amino corrosive. It is trivial in people, which means the body can incorporate it.
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A disulfide bond: they are also called a S-S bond, or disulfide extension, is a covalent bond got from two thiol bunches. In organic chemistry, the wording R-S-S-R availability is generally used to depict the general linkages. The most well-known method for making this bond is by the oxidation of sulfhydryl gatherings.