Assume that you have a 20 M glycerol stick solution. You need to make 5 differen
ID: 484021 • Letter: A
Question
Assume that you have a 20 M glycerol stick solution. You need to make 5 different concentrations of solutions for your experiment. For the experiment you will need to make 50 ml of each concentration of glycerol. Use the formula: C1V1= C2V2Question 1: 10 M glycerol C1- V1- C2- V2-
Question 2: 5 M glycerol C1- V1- C2- V2-
Directions: Assume that you have a 20 M glycerol stock solution. You need to make 5 different concentrations of solutions for your experiment. For the experiment you will need to make 50 ml of each concentration of glycerol. Use the formula: Civi C,V, Final Concentration of Amount (Wi) of 20 M Glycerol Stock Amount of Water Final Volume of the Solution Solution (V2 Solution (C) Needed 0 M glycerol 500 ml 2.5 M glycerol 50 ml 30 ml 5 M glycerol 7.5 M glycerol 50 ml 10 M M glycerol 50 ml Show your calculations for each solution. Remember: As long as the units are the same on both sides of this formula it doesn't matter which units you use, they will cancel out. 0 M glycerol 7.5 M glycerol cm final concentration sourion. Volume of 20 M glycerol needed? Volume of H20 needed? 2.5 M glycerol 10 M glycerol 5 M glycerol
Explanation / Answer
To prepare 50 ml of 10 M glycerol from 20 M glycerol stock solution
V1 = C2V2/C1
with,
C1 = 20 M
C2 = 10 M
V1 = ?
V2 = 50 ml
we get,
volume of 20 M glycerol to be taken = 10 M x 50 ml/20 M = 25 ml
mixed with 25 ml water gives 50 ml solution as needed
To prepare 50 ml of 2.5 M glycerol from 20 M glycerol stock solution
V1 = C2V2/C1
with,
C1 = 20 M
C2 = 2.5 M
V1 = ?
V2 = 50 ml
we get,
volume of 20 M glycerol to be taken = 2.5 M x 50 ml/20 M = 6.25 ml
mixed with 43.75 ml water gives 50 ml solution as needed
To prepare 50 ml of 7.5 M glycerol from 20 M glycerol stock solution
V1 = C2V2/C1
with,
C1 = 20 M
C2 = 7.5 M
V1 = ?
V2 = 50 ml
we get,
volume of 20 M glycerol to be taken = 7.5 M x 50 ml/20 M = 18.75 ml
mixed with 31.25 ml water gives 50 ml solution as needed
To prepare 50 ml of 5 M glycerol from 20 M glycerol stock solution
V1 = C2V2/C1
with,
C1 = 20 M
C2 = 5 M
V1 = ?
V2 = 50 ml
we get,
volume of 20 M glycerol to be taken = 5 M x 50 ml/20 M = 12.5 ml
mixed with 37.5 ml water gives 50 ml solution as needed
for 0 M glycerol all we have to take is 50 ml water