Presented below is the glucose test reaction sequence for the urine reagent test

Question

Presented below is the glucose test reaction sequence for the urine reagent test strips (sticks) used in Lab 6. Please do the following: CIRCLE all the substance(s) in the above reaction scheme that is part of the glucose test strip reagent pad. That is, circle all the substance(s) the manufacturer has placed in or on the reagent pad to make the test work for urine glucose. BOX the substance(s) in the above reaction scheme that give rise to the color end-point interpretable by the unaided eye. Clearly UNDERLINE the substance(s) in the above reaction scheme that comes from the patient urine sample, that is, the substance(s) being measured/detected in a patient's urine sample. Star(*)the substance(s) that confer(s) selectivity for glucose, that is, the reaction sequence component(s) that makes the test

Explanation / Answer

1) substance in glucose reaction pad: Glucose oxidase, peroxidase and KI

2) Substances involved in producing color: H2O2, peroxidase, KI, KIO and I2. The real coloring material is I2

3) Glucose( the first cyclic reactant) comes from patient.

4) Glucose oxidase can selectively oxidizes glucose.

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