The following sentences describe the process of transcription in prokaryotes. Fi

Question

The following sentences describe the process of transcription in prokaryotes. Fill in each blank using the terms provided and then arrange the sentences in the correct order, beginning with the event that occurs first. promoter stop codon DNA polymerase elongation RNA polymerase operator RNA helicase termination initiation start site terminator sequence codon anticodon _______ begins to unwind and separate the 2 strands of the DNA double helix. Actual synthesis of the RNA transcript begins at the . When RNA polymerase reaches a , the RNA transcript and RNA polymerase separate from the DNA. and the DNA within the transcription bubble rewinds. Successive RNA nucleotides are added to the 3 end of the growing RNA chain during a process called . RNA polymerase binds to a region of the DNA called the

Explanation / Answer

1. RNA Polymerase 2. Start Site 3. Terminator Sequence 4. Elongation 5. Promoter

E.coli transcription is initiated by the enzyme RNA polymerase. The polymerase binds specifically at a sequence called promoter. The RNA polymerase requires subunit to recognize and bind strongly at promoter region.

RNA polymerase does not require any primer and can start synthesis of RNA de novo. The synthesis of RNA starts from initiation codon or start site. The transcription occurs in 5’--à 3’direction, that is, new nucleotides are added to the 3’OH of the existing strand. The addition of nucleotides is called elongation. When the polymerase reaches a terminator site, the enzyme stops transcription, get released from DNA and the transcription bubble rewinds.

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