Blood Questions Please Help! a). True or False? Nucleated RBC\'s are lysed and n
ID: 50736 • Letter: B
Question
Blood Questions Please Help!
a). True or False?
Nucleated RBC's are lysed and not counted using the Manual White Blood Cell counting system for WBC
counting.
b). Why is it important to expel the first few drops from the Manual WBC Cell Counting system pipette before plating onto the hemacytometer?
c). When manually making a blood smear and the blood appears to be very thin and runs to the far end of
the slide when you make the slide, how should you alter your slide making technique to make the slide
in an acceptable size?
d). What are the major components of Wright's stain and what staining color does each produce?
e). Which stain do basophils like? Which stain do eosoinphils like? Which stain do neutrophils like?
f).What can happen to blood for hematology work collected in EDTA in the following instances?
A. Blood smear is made 6 hours after collection in EDTA.
B. Tube of blood received is only 1/4 full and the physician insists that work be done on this specimen.
Explanation / Answer
False. Due to their nucleated nature, these cells are not lysed and are thus counted as white blood cells in the WBC counting system.
It is important to expel these drops from the pipette because these drops are part of unmixed and relatively cell free fluid present in the pipette.
Azure B, eosin y and oxidized methylene blue are the main components of Wright's stain. Azure B and methylene blue stain blue to purple colour while eosin Y stains orange to pink color.