Post Laboratory Questions 1 Distilled water frequently contains dissolved (b) Ba
ID: 510610 • Letter: P
Question
Post Laboratory Questions 1 Distilled water frequently contains dissolved (b) Based on your advice in (a, a researcher used Coa, making the pH dinnerent from the theoretical this very butter in a growth medium (including value for pure water. Would this cause the pH of appropriate food and temperature control) for your buffer to be slightly higher or slightly lower bacteria that proliferate at physiological pH Despite than the target pH? Explain. this carefully prepared medum, however, bacterial growth was dismal. Explain. 2. (a) was the pH of the buffer you prepared in their work. Here, "phosphate" refers generaly to slightly higher or slightly lower than the target the class of phosphate derived ions (a) which of these ions best represents the pH? Explain. (b) The pH of a butter can be adjusted by small amounts of NaoH. Which of these should be added to your buffer to remedy the problem identified in (a)? Defend your choice. (b) A butter used in an experiment was described phosphate buffer with pH as "a 0.2 M Based on the conjugate acid you identtied conjugate acid in determine the concentration phosphate buffer. this 0.2 M Consider the HCIo/clo pair, Consult your text or other source for appropriate acid dissociation constants, The ypochlorite ion is the active ingredient in bleach, Nacio(aq). (a) Explain this pair. would be a candidate for a buffer with pH E 7.3 physiological value)Explanation / Answer
Equation is:
CO2 + H2O H2CO3
H2CO3 H+ + HCO3-
So clearly we can say that dissolving CO2 in water will increase the concentration of H+ ion concentration which further decreases the pH of distilled water.
So here we have distilled water whose pH is already slightly lesser than the original.
Thus the prepared buffer solution will have its pH slightly less than the target value.
The remedy is to increase the pH of the buffer solution to reach the target value.
As we know HCl is used for decreasing solution pH and NaOH is used for increasing its pH.
So, for adjusting i.e. increasing the pH of buffer solution, you need add a base like NaOH.