Prelab N9 Enantioselective synthesis by Enzymatic ysis Using Pig Liver Esterase
ID: 518777 • Letter: P
Question
Prelab N9 Enantioselective synthesis by Enzymatic ysis Using Pig Liver Esterase 1. What properties does an ideal esterase enzyme possess? a. Low substrate selectivity b. Produces high stereoselectivity c. Both a and b d. None of the above 2. What role does the enzyme play in the stereospecificity? a. Its active site is the catalyst for chirality. It does not play a role in the chirality. It only speeds up the reaction. ed. Both b and c 3. If the reaction solution is too basic, what would be the possible outcome of the product? a. The reaction would be too hot to generate any product. b. A carboxylate would form instead of the carbokylicacid. y The reaction would not generate any product since the enzyme works better in acidic conditions. d. None of the above. 4. If the reaction solution is too acidic, what would be the possible outcome of the product? a. Both esters would form into carboxylic acids. b. The product would have a high boiling point. c. The product would have a high melting point. d. All of the above 5. Would the theoretical yield be more than, less than, or equal to the original starting reactant? a. More than b. Less than c. Equal d. None of the aboveExplanation / Answer
Ans 1;- An ideal esterase produces high streoselectivity as the enzyme is composed of chiral compound so it can catalyse chemical reaction with high stereoselectivity.
Therefore the correct option is B
Ans 2) Enzyme has a region on its surface called active site which plays as the catalyst for chirality in stereospecificity.As the enzyme is composed of chiral environment so the catalyst enables stereoselective formation.
Therefore the correct option is A
Ans 3;- If the reaction solution is too basic the reaction would not generate any product since the enzyme will work in an ideal pH condition and here the pH condition is changed and as a result the enzyme starts deforming and it does not produces product.
Therefore the correct option is C
Ans 4 . If the reaction solution is too acidic then it will produce high amount of carboxylic acid as then both the esters will undergo acid catalysis in presence of alcohol and hence produces more amount of carboxylic acid.
Therefore the correct option is A
Ans 5) The theoritical yield would be less than the original starting reactant as it will go various transformation such as temperature, pH stability .
Therefore the correct option is B