Above is a chromatogram the first peak represents the time it takes for an un-re

Question

Above is a chromatogram the first peak represents the time it takes for an un-retained substance to pass through the column; it has no interaction with the stationary phase.

1. In the experiment illustrated in Figure 1, which of these variables could change the elution order? Explain your reasoning.

                        Mobile phase composition                   Length of column                       

                        Solvent flow rate                                  Stationary phase composition

2. Suppose you wish to decrease the amount of time for all the peaks to elute but continue to completely separate the compounds in Figure 1. Which of the following variables would affect this change? Explain your reasoning.

Mobile phase composition             Length of column                       

            Solvent flow rate                            Stationary phase composition

3. Which of the variables you identified in question 2 would be easiest to change and give the desired effect?   Explain your reasoning.

Explanation / Answer

Answer:

1)The composition of the mobile phase and solvent flow rate will change of the the elution order.

2)Composition of mobile phase decrease the amount of time for all the peaks to elute but continue to completely separate the compounds

3)The solvent flow rate is easiest to change to give the desired effect

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