Assume you also inoculated a PR base broth (has all the same components as your

Question

Assume you also inoculated a PR base broth (has all the same components as your PR carbohydrate test broth except no carbohydrates are added) along with your regular PR carbohydrate test tubes. Looking at the results listed in the table below, state which results would be reliable and give one plausible explanation for each unreliable result (no need to provide explanation for reliable results).

PR (carbohydrate added)

PR base (no carbohydrate added)

Reliable test? (Y/N)

Explanation

A/G

A/G

A/G

-/-

A/G

K

A/G

A/-

K

K

K

-/-

A/-

K

A/-

A/G

PR (carbohydrate added)

PR base (no carbohydrate added)

Reliable test? (Y/N)

Explanation

A/G

A/G

A/G

-/-

A/G

K

A/G

A/-

K

K

K

-/-

A/-

K

A/-

A/G

Explanation / Answer

PR (carbohydrate added)

PR base (no carbohydrate added)

Reliable test? (Y/N)

Explanation

A/G

A/G

A/G

-/-

A/G

K

A/G

A/-

K

K

K

-/-

A/-

K

A/-

A/G

PR (carbohydrate added)

PR base (no carbohydrate added)

Reliable test? (Y/N)

Explanation

A/G

A/G

Not reliable because no acid and gas produced when carbohydrate is absent in PR base

A/G

-/-

Reliable

A/G

K

Reliable

A/G

A/-

Not reliable No gas can produce in the absence of carbohydrates

K

K

Reliable

K

-/-

Reliable

A/-

K

Reliable

A/-

A/G

Not reliable No acid and gas formation occurs in the absence of carbohydrates

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