Question
In eukaryotic genes the noncoding regions are called _______. During translation, the for the amino acid _____ serves as the start signal. (Name the amino acid in either prokaryotes or eukaryotes for credit). Amyloid protofibrils contain ordered arrays of ________ secondary structures. The absorbance of a protein solution was measured to be 5.6 at 280 mm. The extinction coefficient (epsilon) for the protein is .70 cm^2 mg^ and the absorbance was measured in a cuvette with a pathlength of 0.65 cm. What is the concentration of the protein?_________. Scurvy is due to _______ which causes weakening of collagen fibers due to failure to hydroxylate the sidechains of ______
Explanation / Answer
Q9.
A = 5.6
e = 0.70
l = 0.65 cm
then, find C:
apply beer law:
A = E*l*C
note thate C is in mg / cm3 accorindg to E
substitute calues
5.6 = 0.70 * 0. 65 * C
C = 5.6 / (0.70*0.65) = 12.30 mg/cm3