Problem 12.1 in the TLC Study Problem section of the Chromatography Ch of the Ha
ID: 525713 • Letter: P
Question
Problem 12.1 in the TLC Study Problem section of the Chromatography Ch of the Handbook for Organic Chemistry Lab. What could happen if you used ink to draw in your base line and letters TLC plate? What could happen if you spot too large an amount of a compound on the plate? What would happen if your solvent level is above the level of the initial The CRC lists the melting point for a compound as 182-183 degree C. You melting point for this same compound isolated in your experiment as 181 degree C. What can you conclude about the compound isolated in yourExplanation / Answer
TLC
2) Ink contains organic compounds which If used to spot line of tlc, would also develop in the solvent and interfere and mess up the tlc of the actual mixture to be detected.
3) If large amount of sample is used for spotting on the tlc plate, the spots would appear as large blots as it spread on the plate and the Rf calculation from it would be inaccurate.
4) If solvent level is above the level of spots, the compund would dissolve even before the tlc starts to run and no more compound or incorrect Rf values would be obtained.
5) The melting point observed by experiment is close to the literature melting point. The compound is thus pure.