All of the following arc intermediates in the glycolysis pathway EXCEPT: A. Gluc

Question

All of the following arc intermediates in the glycolysis pathway EXCEPT: A. Glucose 3-phosphate B. 3-phosphogIycerate C. 2-phosphoglycerate D. Fructose 6-phosphate An enolase is an enzyme that is responsible for catalyzing a (n) ___ reaction. If 8 molecules of glucose enter into the glycolytic pathway, what is the total NET ATP that can be made. Glycolysis an an ___ pathway that takes place in the ___ of the cell. A. Acrobic, Cytoplasm B. Anaerobic. Mitochondrial Matrix C. Aerobic, Mitochondrial Intermembrane Space D. Anaerobic, Cytoplasm What are the names of the four compartments of the mitochondria If there is no oxygen available in yeast, pyruvate is converted into which of the following A. Acetyl-CoA B. Ethanol C. Lactate D. Glucose All of these items are produced in the Citric Acid Cycle EXCEPT: A. CO_2 B. FADH_2 C. NADH D. Acetyl Co-A

Explanation / Answer

Question 1. Answer: A. Glucose 3-phosphate

In glycolysis pathway glucose is first converted into glucose 6-phosphate using ATP (Adenosine TriPhosphate) which is converted to ADP (Adenosine DiPhosphate). Glucose 6-phosphate undergoes rearrangement and another phosphorylation reaction to form fructose 1,6-biphosphate. Cleavage of fructose 1,6-biphosphate leads to formation of glyceraldehyde 3-phosphate. Hence, glucose 3-phosphate is NOT an intermediate in glycolysis pathway.

Question 2. Answer: enolization

In 9th step of glycolysis pathway enolase catalyzes conversion of 2-phosphoglycerate into phosphoenol pyruvate.

Question 3. Answer: 64 ATP

When one glucose molecule enters glycolytic pathway, it can lead to formation of net 8 units ATP. Totally 2 units of ATP are consumed in steps 1 and 3.In step 6, 6 units of ATP are formed; in each of steps 7 and 10, 2 units of ATP are formed. So net ATP formed per glucose molecule is -1 -1 + 6 + 2 + 2 = 8. Hence, 8 glucose molecules lead to formation of 64 units of ATP.

Question 4. Answer: A. Aerobic, cytoplasm

Glycolysis takes place in cytosol, the intracellular liquid that surrounds organelles in cell. In glycolysis, glucose is converted to pyruvate. This transformation takes place in 10 steps and molecular oxygen is not required in any of these steps. Glycolysis is said to be aerobic if it takes place in presence of oxygen. The oxygen is used to oxidize NADH produced in step 6 and for oxidation of pyruvate produced in step 10. In aerobic glycolysis net 8 ATP units are formed per glucose unit. In anaerobic glycolysis ( when sufficient oxygen is not available) net 2 ATP units are formed per glucose unit since NADH produced in step 6 is used for converting pyruvate to lactate.

Question 5. Answer: The four compartments of mitochondria are,

1. Outer membrane 2. Intermembrane space 3. Inner membrane 4. Matrix

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