This Table shows assay data from a purification procedure. Which statement is mo
ID: 533146 • Letter: T
Question
This Table shows assay data from a purification procedure. Which statement is most likely TRUE? The gel-filtration step increased the specific activity but not the yield. Of the three chromatography steps, the affinity column step had the biggest drop in yield. After the CM-cellulose step, the purification factor reached over 1000. indicating effective purification. Most of the protein removed in the gel- filtration step was likely enzyme that had unfolded during the CM-cellulose step. All of the above are false.Explanation / Answer
Given the data for M4-HW-07,
the specific acivity and total protein content,
The true statement about purification procedure based on the given data would be,
A3. after the CM-cellulose step, the purification factor reaches over 1000, inidicating effecive purification.