Part Ill: Sample Holders Consider this... Figure 6 Transmission materials used f

Question

Part Ill: Sample Holders Consider this... Figure 6 Transmission materials used for cells, windows, lenses in UV/Vis spectrophotometers Republication permited courtesy of Intermational Crystal Laboratories) spectrum of fused silica and borosilicate crown glass (BK7), two common Fused Silica Sio, (Ultraviolet Gradel Wavelength in Microns Borosilicate Crown Glass (BK7 Wavelength in Microns 24. The UV/Nis region of the electromagnetic spectrum is from 200-800 nm. Label this region on each plot in Figure 6, (NOTE: microns are another word for micrometers, 25. Examine Figure 6. What wavelengths of light are not absorbed by fused silica ti.e. they are transmitted), and why would fused silica be a good material to use in a sample holder for a UVVis spectroscopy experiment? 26. What wavelengths of light transmit through borosilicate crown glass (BK7), and at what wavelengths would this material not be useful for a UVNis absorption measurement 218 144

Explanation / Answer

24. 200 to 800 nm means 0.2 to 0.8 microns (in this region transmittance is parallel)

25. Fused silica is not absorbing from 210 nm to 1000 nm (since usual UV/vis region is coming in this range, fused silica can be a better material to use for absorption measurements).

26. Below 320 nm, in UV region it is not advisable since it has an absorbance and above 320 to 1000 nm it can be used.

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