Can you help me with my Final Applied Lab Project for Biology103? How can I crea
ID: 55607 • Letter: C
Question
Can you help me with my Final Applied Lab Project for Biology103?
How can I create an experiment testing the effects of low pH on enzyme activity?
Help! I’m supposed to do an experiment, but I’m kind of confused. I have five test tubes, and I’m supposed to be testing the effects of low pH on enzyme activity. I’ve chosen Amylase/banana as my enzyme and starch/potato as my substrate. (I also have left over starch liquid and IKI.) I was going to test the different pHs by adding water (7), draino (14), soda (4), banana (5) and soda (4) to 1 cubic centimeter of a potato in each tube. I guess I’m just not sure if I’m doing it right.
The independent variable influences the change in my dependent variable. Is my dependent variable the potato? My independent variable would be the water, draino, soda, banana and soda. What would my control be? Thank you for your help.
Am I supposed to put 1 cubic centimeter of potato in each test tube and then only put the banana in one test tubes with the potato or get rid of the potato and only use the banana in each test tube? Sorry, I'm confused.
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. ID the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH.
Explanation / Answer
Enzymes are mostly proteinaceous in nature. They are affected by changes in pH. The most favourable pH at which the enzyme is most active is known as the optimum pH. pH values other than optimum pH leads to reduction in enzyme activity. Extremely high or low pH values may cause the enzyme to completely loss its activity.
Principle of our experiment :
Amylase is an enzyme that acts on starch (substrate). 1% Iodine solution is employed to test the activity of enzyme. Starch reacts with iodine solution to give blue black coloration. Hence we will observe the color change.
Starch + I2 à blue-black color
APPARATUS
Equipment
Effect of pH
Observations :
pH range 6.5 to 7.5 will give clear solutions since it is an optimum pH range for amylase, hence it will completely degrade the starch present in the test tube.
Other pH values 4.5-6.0 = brown-red colored solutions since enzyme will degrade starch partially
2.5-4.5 pH value = deep blue color solutions obtained since enzyme will be completely denatured and has no activity, thus the solution contains undigested starch which will react with iodine solution to give deep blue color